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Functional validation of sorted fractions was carried out by xenotransplantation, the result of which was coupled with miRNA expression profiling to deliver stem mobile-connected miRNA expression profiles.
To determine whether or not miRNA are differentially expressed in LSC and HSC, we fractionated sixteen AML individual samples and a few lineage-depleted (Lin–) cord blood (CB) samples applying CD34 and CD38 into four populations and subjected each to world-wide miRNA profiling; the stem mobile material of each and every portion was functionally assayed by xenotransplantation (Figures 1A and S1A).
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Reducing the miR-126 level impairs LSC maintenance in contrast to expanding regular hematopoietic stem cells.
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The LSC frequency was lessened in two of 3 samples on 126KD (Figures 5C and S5D). Jointly, these conclusions advise that 126KD generates heterogeneous responses with LSC operate and frequency minimized in a subset of AML patients.
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(E) Per cent BrdU incorporation into bulk cultures exhibiting proliferation of CTRL and 126OE transduced 8227 cells after some time.
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